Predicting biomolecule adsorption onto chromatography resins remains a key challenge in biopharmaceutical separations. A thorough mechanistic understanding of adsorption is crucial for optimizing chromatographic performance. Thermodynamic studies, through batch equilibrium experiments and calorimetric analyses, have been instrumental in unraveling complex adsorption mechanisms, with microcalorimetry emerging as an essential tool in preparative chromatography. In particular, flow microcalorimetry (FMC) has proven to be a powerful technique for directly tracking heat signals associated with adsorption and desorption events, under both linear and overloaded conditions. By capturing dynamic thermal profiles, FMC enables characterization of biomolecule – chromatography support interactions, offering real-time insights into biomolecule-resin interactions and aiding in process optimization and scale-up. This workshop will showcase the technical capabilities of FMC alongside other calorimetric methods, including isothermal titration calorimetry (ITC) and differential scanning calorimetry (DSC), illustrating how these techniques enhance process understanding, improve robustness, and accelerate biopharmaceutical development. Emphasis will be placed on FMC as an in-situ approach closely reproducing actual chromatographic conditions. Practical applications in antibody, peptide, and polynucleotide separations using different resins (hydrophobic, ion-exchange, affinity and silica-based) will be discussed.